Volume 54, Issue 2, 27 January 2026

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NAR Breakthrough Article

Synthesis of long and functionally active RNAs facilitated by acetal levulinic ester chemistry

Zidi Lyu and others

Disruption of a six-nucleotide miRNA motif improves PKD1 dosage and ameliorates polycystic kidney disease

Ronak Lakhia and others

Critical Reviews and Perspectives

Stability of non-canonical nucleic acid structure as a potential modulator of cell fate

Shuntaro Takahashi and others

Illuminating the genome: emerging approaches in CRISPR-Cas live-cell imaging

Zhiguang Xiao and Yujie Sun

Chemical Biology and Nucleic Acid Chemistry

Triple base editor catalyzes saturation mutation of adenine, cytidine, and guanine

Youming Wu and others

PolyA tail segmentation improves the stability of the template DNA and increases the translatability of in vitro transcribed mRNA

Tomasz Spiewla and others

The two-step purification method ViREn identifies a single NSUN6-mediated 5-methylcytosine modification promoting dengue virus RNA genome turnover

Chia Ching Wu and others

Computational Biology

TiRNA: a coarse-grained method with temperature and ion effects for RNA structure folding and prediction

Xunxun Wang and others

Deciphering the 3D genome organization across species from Hi-C data

Aleksei Shkolikov and others

Data Resources and Analyses

(Re)defining the human chromatome: an integrated meta-analysis of localization, function, abundance, physical properties, and domain composition of chromatin proteins

Anna K Gribkova and others

An AI-guided framework reveals conserved features governing microRNA strand selection

Dalton Meadows and others

Gene Regulation, Chromatin and Epigenetics

Bacteria sense the antibiotic rifampicin through a widespread dual-promoter based alarm system

Petra SudzinovÃ¡ and others

Dps binds and protects DNA in starved Escherichia coli with minimal effect on chromosome accessibility, dynamics, and organization

Lauren A McCarthy and others

E2F1 K117 methylation by SETD6 disrupts BRD4'E2F1 binding and modulates E2F1 chromatin binding and gene regulation in prostate cancer cells

Gizem Tugce Ulu and others

G34R cancer mutation alters the conformational ensemble and dynamics of the histone H3.3 tails

Harrison A Fuchs and others

An EGR1-dependent cascade modulates genome architecture at the CSF1R locus

Sandra Deliard and others

NF-kappa B restrains nutrient-dependent transcription programs through chromatin modulation in Drosophila

Xiangshuo Kong and others

Comprehensive profiling of chromatin occupancy dynamics through the cell cycle

Yulong Li and others

eIF3g binding to GUCG boxes located in mRNA coding regions enhances translation of mild heat shock response genes in the yeast Saccharomyces cerevisiae

Hiroaki Kato and others

A noncanonical transcriptional regulation in natural product biosynthesis

Jinjin Wang and others

YhfH functions as a tri-function RNA in Bacillus thuringiensis BMB171

Jiaxin Qin and others

Distal enhancers regulate mammalian early embryonic lineage differentiation through long-range interactions

Qianshu Zhu and others

Genome Integrity, Repair and Replication

Human cytomegalovirus regulates host DNA repair machinery for viral genome integrity

Pierce Longmire and others

Effects of ribonucleotides on telomeric G4 formation, dynamics, and initiation of ribonucleotide excision repair by RNase H2

Luis M Cortez and others

Safeguarding genome integrity: Polo-like kinase Cdc5 and phosphatase Cdc14 orchestrate Topoisomerase II-mediated catenane resolution in mitosis

Lucia F Massari and others

Linking kinetochore attachment to checkpoint control: the role of Aurora B in BubR1 acetylation

Si-Young Choi and others

Visualization and quantification of rDNA instabilities in mammalian cells and mouse models

Xiaolu Zhu and others

NSMF modulates replication stress to facilitate colorectal cancer progression

Kyeong Jin Shin and others

The SMC5/SMC6 complex is critical for resolving R-loop-induced transcription'replication conflicts

Tong Wu and others

Termination of DNA replication drives genomic instability via multiple mechanisms

Daniel J Goodall and others

Microhomology-mediated end joining is the predominant form of DNA repair in the mosquito Aedes aegypti with implications for gene editing, gene drive, and transgene removal

Joseph S Romanowski and others

Dynamic DnaA'DnaB interactions at oriC coordinate the loading and coupled translocation of two DnaB helicases for bidirectional replication

Takumi Tsuruda and others

Mbd4 and MutSalpha protect cells from spontaneous deamination of 5-methylcytosine

Rebecca A Bilardi and others

REV7 associates with ATRIP and inhibits ATR kinase activity

Megan Biller and others

Genomics

Comparative mitogenomics of kingdom Fungi ' evolutionary insights and metagenomic applications

Steven R Ahrendt and others

Host range and antibiotic resistance dissemination are shaped by distinct survival strategies of conjugative plasmids

Ryuichi Ono and others

Molecular and Structural Biology

Mrx6 binds the Lon protease Pim1 N-terminal domain to confer selective substrate specificity and regulate mtDNA copy number

Simon Schrott and others

Structural insights into the Bre1'Lge1 and RNF20/RNF40'WAC interactions critical for H2B ubiquitination

Meng Shi and others

Structure and function of the nairovirus cap-snatching endonuclease

Wenhua Kuang and others

Strategic variations in sarbecovirus and merbecovirus Nsp1 linker regions for translation inhibition

Ruixi Yan and others

Solid-state nanopore sensing reveals conformational changes induced by a mutation in a neuron-specific tRNA^Arg

Shankar Dutt and others

DNA binding and dimerization of the SOG1 NAC domain are functionally linked with its ability to undergo liquid'liquid phase separation

Kim Mignon and others

A fold switch regulates conformation of an alphavirus RNA-dependent RNA polymerase

Jamie J Arnold and others

Nucleic Acid Enzymes

Metal ions govern coronavirus endoribonuclease activity

Xionglue Wang and others

Single-molecule experiments reveal NusG displaces transcription initiation factor Ïƒ⁷⁰ from mature elongation complexes

Pratip Mukherjee and others

Disordered DNA-binding motif forms a modulation site for inhibiting the cancer immunotherapy target TREX1

Kuan-Wei Huang and others

A minimal RNA-cleaving DNAzyme and its catalytic mechanism

Kazuhiko Yamasaki and others

Machine learning reveals sequence and methylation determinants of SaCas9'PAM interactions in bacteria

Dalton T Ham and others

Tho1 and MOS11 promote nucleic acid double-strand unwinding by facilitating DEAD-box helicase oligomerization

Fabienne Becker and others

Bacteriophage deploys a RecA-dependent nuclease to inhibit Staphylococcus aureus replication and promote phage propagation

Qi Xu and others

The transitional kinetics between open and closed Rep structures can be tuned by salt via two intermediate states

Jamieson A L Howard and others

Human mitochondrial helicase Twinkle has RNA binding, annealing, and strand-exchange activities

Anupam Singh and others

Nucleic Acid Therapeutics

RNA editing for the treatment of alpha-1 antitrypsin deficiency

Prashant Monian and others

Compact bacterial recombination complexes drive efficient kilobase-scale knock-in in mammalian cells

Yining Luo and others

RNA and RNA-protein complexes

Derepression of a single microRNA target causes female infertility in mice

Joanna Stefano and others

A transcriptomic and proteomic map of primary human cell types

Dong-Gi Mun and others

TlyA is a 23S and 16S 2²-O-methylcytidine methyltransferase important for ribosome assembly in Bacillus subtilis

Jennie L Hibma and others

RNA G-quadruplexes promote codon repeat-associated ribosomal frameshifting in human genes

Xiuwen Li and others

The long noncoding RNA VIM-AS1 and nucleoporin Nup358/RanBP2 regulate SMAD nuclear accumulation during TGF-beta signaling

Dorival Mendes Rodrigues-Junior and others

The HigB-like SehA toxin promotes non-replicating Salmonella inside macrophages by inhibiting ribonuclease III-dependent rRNA maturation

Soomin Choi and others

Synthetic Biology and Bioengineering

Multilayered regulation by RNA thermometers enables precise control of Cas9 expression in E. coli

Elise K Kammerdiener and others

Steric regulation of CRISPR/Cas12a trans-cleavage kinetics via split-activator extensions

Jianhong Zhang and others

Methods

Optogenetic BlueGENEs engineered into a human safe harbor locus

Alexander Geidies and others

RaptScore: a large language model-based algorithm for versatile aptamer evaluation

Akira Kimura-Yamazaki and others

RoCi'a single-step multi-copy integration system based on rolling-circle replication

Martzel Antsotegi-Uskola and others

Interpretable trajectory inference with single-cell linear adaptive negative-binomial expression (scLANE) testing

Jack R Leary and others

Optimized CRISPR-Cas9 system for efficient engineering of ecDNA in cancer cells

Yohei Sugimoto and others

Template nicking suppresses promoter-independent antisense transcription in IVT via R-loop-mediated strand displacement

Goeun Lee and others

A predictive framework for stop-loss variants with C-terminal extensions

Jihoon G Yoon and others

Corrections

Correction to ˜inDrops-2: a flexible, versatile and cost-efficient droplet microfluidic approach for high-throughput scRNA-seq of fresh and preserved clinical samples'

Correction to ˜A quorum sensing-controlled type I CRISPRi toolkit for dynamically regulating metabolic flux'

Correction to ˜Single-cell imaging of N⁴-acetylcytidine-modified RNA using fluorine metabolic labeling mediated proximity ligation assay'

Correction to ˜DMRdb: a disease-centric Mendelian randomization database for systematically assessing causal relationships of diseases with genes, proteins, CpG sites, metabolites and other diseases'

Correction to "Ubiquitin ligase UBR3 regulates cellular levels of the essential DNA repair protein APE1 and is required for genome stability"

Correction to "ARF induction in response to DNA strand breaks is regulated by PARP1"

Correction to ˜MetaflowX: a scalable and resource-efficient workflow for multi-strategy metagenomic analysis'

Correction to ˜Heterochromatome wide analyses reveal MBD2 as a phase separation scaffold for heterochromatin compartmentalization and composition'

Correction to: HLRMDB: a comprehensive database of the human microbiome with metagenomic assembly, taxonomic classification, and functional annotation by analysis of long-read and hybrid sequencing data

Editor's Note

Editor's Note on ˜Human base excision repair complex is physically associated to DNA replication and cell cycle regulatory proteins'

This email is intended for bionet@evol.mcmaster.ca.